SARS-CoV-2 Spike Protein
To share with any labs in TAIWAN which are doing SARS-CoV-2 research
"Open Science" approach has been adapted around the globe in the fight against SARS-CoV-2, here we are happy to share high-quality, human-cell-expressed SARS-CoV-2 spike protein FOR FREE, in order to contribute to Taiwan's national research efforts in detection, treatment and prevention of SARS-CoV-2 (2019-nCoV, or Wuhan virus; the causative agent of COVID-19).
The spike protein consists of the whole extracellular domain including S1 (with RBD) and S2, from residue 14 to 1209, no transmembrane domain, with an apparent molecular weight between 170 to 235 kDa on SDS-PAGE (monomer), and ~600 kDa (trimer) on Superose 6 size-exclusion chromatography. It is expressed in HEK 293 cells with human complex type glycans, and exists as a trimer in solution.
於新冠肺炎大流行期間，本於開放式科學的精神，本實驗室將無償分享人類細胞純化之 SARS-CoV-2 刺突蛋白 (Spike protein) 於全台灣研究單位，以促進台灣在檢測、治療和預防 SARS-CoV-2（2019-nCoV 或武漢病毒: COVID-19的病原體）之發展。
本實驗室所純化之刺突蛋白長度為胺基酸列14到1209，於 SDS-PAGE 上的分子量為170-235 kDa 之間，分子篩層析 (Superose 6 ) 的分子量在〜600 kDa（三聚體）。蛋白所帶有的醣基化醣類為複合型醣類，並以三聚體形式存在於水溶液中 。
March 31, 2020 updated
The SARS-CoV-2 research is supported by "Novel Therapeutics for Drug-Resistant Infectious Diseases" of the Summit Project (AS-SUMMIT-109 登峰計畫), Genomics Research Center, Taiwan Academy of Sciences.
The spike protein is provided as is. It is aliquoted, snap-frozen in liquid nitrogen and stored in -80 degree. For more info, click here.
This protein is of limited quantity and we cannot guarantee availability upon request, but we will send notification once it's back in stock.
You need to arrange in advance to come pick up in person on the thrid floor of Genomics Research Center in Academia Sinica.
If you use it in any of your publication, please acknowledge: Ting-Hua Chen, Xiaorui Chen and Jennifer M. Lo, for their effort in preparation for the SARS-CoV-2 spike protein since February 2020.
If you need more than 50 ug, we will be happy to talk about the possibility of collaboration.
*Freeze-dried sample can be mailed at room temperature to any place in Taiwan and dissolved in water ready for use. Its quality compared to fresh frozen sample has not been tested, but it should be fine if only used in ELISA and western blot.